Nutritional Analysis
and Food Labelling

We provide accredited nutritional analysis for FDA Nutrition Facts labels. Specifically, our PJLA ISO/IEC 17025:2017 scope covers minerals (Sodium, Calcium, Iron, Potassium), cholesterol, caffeine, protein, fat, fatty acids, dietary fiber, total sugars, and Vitamins A, C & D.

Accreditation
ISO/IEC 17025:2017 · PJLA #117946
Certificate
Cert L26-535
Techniques
ICP-MS · GC-FID · HPLC · Titrimetric
Accredited Analytes
Minerals, Cholesterol, Caffeine, Protein, Fat, Fiber, Sugars, Vitamins A/C/D
Turnaround
5–7 Business Days

Accredited Nutritional Analysis for FDA Nutrition Facts Labels

Accurate nutritional analysis and food labelling is essential for regulatory compliance under the US FDA's Nutrition Labelling and Education Act (NLEA, 21 CFR 101.9). Consequently, AGT Food Labs' PJLA ISO/IEC 17025:2017 scope (Cert L26-535) covers most of the mandatory Nutrition Facts nutrients that require laboratory measurement. Specifically, this includes the four label minerals (Sodium, Calcium, Iron, Potassium), plus Cholesterol, Protein, Total Fat, Saturated & Trans Fat (fatty-acid profile), Total Dietary Fiber, Total Sugars, and Vitamins A, C, and D. In addition, we quantify Caffeine for voluntary label disclosure on beverages and supplements.

To ensure accuracy, we deliver this data through accredited methods. For instance, we utilize ICP-MS for the mineral panel (AOAC 985.01 / 993.14, SOP 047), GC-FID for cholesterol (AOAC 994.10, SOP 080) and the fatty-acid profile (AOAC 996.06, SOP 079), HPLC for caffeine (AOAC 979.08 / ISO 20481, SOP 048), titrimetric determination for protein (AOAC 2001.11, SOP 068), gravimetric fat (AOAC 922.06, SOP 069), enzymatic-gravimetric dietary fiber (AOAC 985.29, SOP 071), and HPLC for total sugars and vitamins. Meanwhile, Calories and Total Carbohydrate are calculated from these measured values per FDA convention, while Added Sugars is documented from your formulation and ingredient records, since it cannot be measured analytically. Furthermore, you can round out your label compliance with food allergen testing and also verify food additives for clean-label declarations.

Why Nutritional Labelling Matters

Building consumer trust through transparent nutrient reporting.

Regulatory Compliance

First and foremost, adhere to FDA 21 CFR 101.9 standards for nutritional analysis and food labelling using accredited nutrient testing.

Health Claims

Additionally, scientifically validate marketing claims such as "Low Sodium," "Low Cholesterol," "High Protein," or "Excellent Source of Fiber" with defensible label data.

Reformulation

Moreover, track the impact of recipe changes on minerals, cholesterol, caffeine, fat, protein, sugars, and fiber during product development.

Accredited Services for Nutritional Analysis and Food Labelling

Mineral panel by ICP-MS, cholesterol by GC-FID, and caffeine by HPLC — all under our PJLA ISO/IEC 17025:2017 scope.

Mineral Panel by ICP-MS for Nutrition Facts Labels

ICP-MS instrument running accredited mineral panel for nutritional analysis and food labelling per AOAC 985.01 / 993.14

Specifically, our ICP-MS mineral panel under AOAC 985.01 / 993.14 (SOP 047) quantifies the four mandatory minerals on the Nutrition Facts panel—Sodium, Calcium, Iron, and Potassium. In addition, it measures nine further elements widely used for fortification verification and quality control. Furthermore, you can validate nutrient stability over time with shelf life label claims.

Elements Reported

Sodium (Na), Potassium (K), Calcium (Ca), Iron (Fe), Magnesium (Mg), Zinc (Zn), Copper (Cu), Manganese (Mn), Selenium (Se), Chromium (Cr), Aluminium (Al), Tin (Sn), Nickel (Ni).

Method

AOAC 985.01 / 993.14 by ICP-MS (SOP 047), under PJLA ISO/IEC 17025:2017 accreditation, Cert L26-535. Additionally, SOP 051 (AOAC 2015.06, Microwave Digestion ICP-MS) extends the panel to include Molybdenum (Mo).

Technical Specs

Sample Size: 50–100 g | LOQ: < 1 ppm (varies by element) | Shipping: Ambient

Turnaround Time

5–7 Business Days · Rush available on request

Typical Use Cases

These include FDA Nutrition Facts label generation, "Low Sodium" / "High Iron" / "Excellent Source of Calcium" claim substantiation, fortified product verification, sports nutrition and dietary supplement potency confirmation, as well as reformulation studies.

Cholesterol Testing by GC-FID

GC-FID cholesterol analysis for FDA Nutrition Facts label declaration at AGT Food Labs

Cholesterol is a mandatory declaration on the FDA Nutrition Facts panel, reported in milligrams per serving. Accordingly, we measure it directly by GC-FID under AOAC 994.10 (SOP 080) rather than estimating it from database values. In parallel, the AOAC 996.06 fatty-acid profile (SOP 079) resolves saturated and trans fat, so your fat and cholesterol lines come from separately accredited determinations.

Method

Cholesterol — AOAC 994.10 (SOP 080, GC-FID). Companion fatty-acid profile for saturated and trans fat — AOAC 996.06 (SOP 079, GC-FID). Both under PJLA ISO/IEC 17025:2017 accreditation, Cert L26-535.

Technical Specs

Sample Size: 100–200 g | Matrices: Meat and poultry, dairy, eggs and egg products, bakery, oils and fats, prepared meals | Shipping: Chilled or frozen for perishables

Turnaround Time

5–7 Business Days · Rush available on request

Typical Use Cases

These include mandatory cholesterol declaration on Nutrition Facts panels, "Low Cholesterol" and "Cholesterol Free" claim substantiation under 21 CFR 101.62, egg and dairy replacement verification, and reformulation studies targeting reduced cholesterol.

Caffeine Quantification by HPLC

HPLC caffeine quantification for beverage and energy product label disclosure at AGT Food Labs

While caffeine is not a mandatory Nutrition Facts nutrient, it must appear in the ingredient statement when added, and many brands voluntarily state milligrams per serving. Therefore, our accredited HPLC method quantifies caffeine so that voluntary disclosures, decaffeinated claims, and batch consistency checks all rest on defensible data.

Method

AOAC 979.08 / ISO 20481 by HPLC (SOP 048), under PJLA ISO/IEC 17025:2017 accreditation, Cert L26-535.

Technical Specs

Sample Size: 50–100 g or 250 mL | Matrices: Coffee, tea, carbonated and energy beverages, chocolate, dietary supplements | Shipping: Ambient

Turnaround Time

5–7 Business Days · Rush available on request

Typical Use Cases

These include voluntary "mg per serving" caffeine declarations, decaffeinated and "Naturally Caffeine-Free" claim verification, batch-to-batch consistency in energy drink production, and formulation control during product development.

Macronutrients & Vitamins Panel

Accredited macronutrient and vitamin analysis for FDA Nutrition Facts labels

Beyond minerals, cholesterol, and caffeine, our PJLA ISO/IEC 17025:2017 scope covers the core macronutrients and vitamins needed to build a complete FDA Nutrition Facts panel. As a result, each analyte below is reported under accredited methods.

Accredited Analytes & Methods

Protein — AOAC 2001.11 (SOP 068, Titrimetric); Total Fat — AOAC 922.06 (SOP 069, Gravimetric); Saturated & Trans Fat — fatty-acid profile AOAC 996.06 (SOP 079, GC-FID); Total Dietary Fiber — AOAC 985.29 (SOP 071, Enzymatic-Gravimetric); Total Sugars — SOP 058 (HPLC); Moisture & Ash — AOAC 925.10 / 920.153 (Gravimetric); Salt — SOP 061 (USDA-FSIS CLG-SLT.04); Vitamins A, C & D — AOAC 992.06 / 2012.22 / 2016.05 (SOP 082/084/083, HPLC).

Calculated & Records-Based Values

Consequently, Calories (Atwater) and Total Carbohydrate (by difference) are derived from the measured analytes above per FDA 21 CFR 101.9 convention rather than tested directly. Added Sugars cannot be measured analytically — no laboratory method distinguishes added from naturally occurring sugars — so that value is documented from your formulation and ingredient records.

Accreditation & TAT

PJLA ISO/IEC 17025:2017 (Cert L26-535) | 5–7 Business Days

Typical Use Cases

Similarly, typical applications include full Nutrition Facts panel generation, "High Protein" / "Good Source of Fiber" / "Low Fat" claim substantiation, and vitamin fortification verification.

Industries We Serve

Nutritional analysis and food labelling solutions across every food sector.

Packaged food samples for nutrition panel testing

Packaged Foods

First, we provide full nutrient and cholesterol reporting for snacks, frozen meals, and ready-to-eat products requiring an FDA Nutrition Facts label.

Beverage samples for caffeine and mineral testing

Beverages & Energy Drinks

Next, we deliver caffeine, sodium, potassium, and sugar quantification for coffee, tea, sports drinks, and energy beverages, thereby supporting label claims.

Dietary supplements

Dietary Supplements

Furthermore, we verify mineral, vitamin, and caffeine potency in capsules, powders, and gummies — specifically Calcium, Iron, Zinc, Magnesium, Selenium, and Vitamins A/C/D.

Bakery products

Bakery & Cereals

Finally, we offer protein, fiber, cholesterol, and iron/calcium fortification verification in breads, fortified cereals, and bakery mixes for defensible nutrition panels.

Technical Capability

Accredited platforms purpose-built for label-grade nutrient data.

ICP-MS, GC-FID and HPLC laboratory equipment used for nutrition analysis
  • ICP-MS — Minerals (AOAC 985.01 / 993.14, SOP 047)

    Specifically, Inductively Coupled Plasma Mass Spectrometry detects thirteen elements in a single run with parts-per-billion sensitivity. Therefore, it performs well below the levels required for FDA Nutrition Facts label declarations.

  • GC-FID — Cholesterol & Fatty Acids (AOAC 994.10 / 996.06)

    In addition, Gas Chromatography with Flame Ionization Detection measures cholesterol directly under SOP 080 and resolves the full fatty-acid profile under SOP 079. Consequently, saturated fat, trans fat, and cholesterol are each reported from accredited determinations.

  • HPLC — Caffeine, Sugars & Vitamins

    Moreover, High Performance Liquid Chromatography quantifies caffeine under AOAC 979.08 / ISO 20481 (SOP 048), total sugars under SOP 058, and Vitamins A, C, and D under AOAC 992.06 / 2012.22 / 2016.05.

  • Quality Control by Method Standards

    To maintain standards, every batch is run with method-blank, fortified spike recovery, and certified reference material (NIST or equivalent). Consequently, we confirm accuracy under PJLA ISO/IEC 17025:2017 accreditation.

Partner with AGT Labs

From formulation to shelf, we deliver the accredited nutrient data your nutritional analysis and food labelling program needs.

★

ISO/IEC 17025:2017 Accredited

To start, nutrient analysis runs under PJLA ISO/IEC 17025:2017 (#117946, Cert L26-535) using AOAC methods for minerals, cholesterol, caffeine, protein, fat, fiber, sugars, and vitamins.

⚡

Regulatory Expertise

Moreover, we help interpret nutrient results in the context of FDA NLEA labelling rules and 21 CFR 101.9 nutrient declaration tolerances.

👥

Formulation Support

Finally, we identify nutrient gaps or excesses to refine your recipe. As a result, this is especially useful for sodium reduction, cholesterol reduction, and protein or fiber enrichment.

Common Questions About Nutritional Analysis and Food Labelling

What does AGT cover under accredited nutritional analysis and food labelling?
To begin with, our PJLA ISO/IEC 17025:2017 scope (Cert L26-535) covers the mineral panel by ICP-MS (AOAC 985.01 / 993.14, SOP 047). In addition, it includes Cholesterol (AOAC 994.10), Caffeine (AOAC 979.08 / ISO 20481), Protein (AOAC 2001.11), Total Fat (AOAC 922.06), Saturated & Trans Fat via fatty-acid profile (AOAC 996.06), Total Dietary Fiber (AOAC 985.29), Total Sugars (SOP 058), and Vitamins A, C, and D (AOAC 992.06 / 2012.22 / 2016.05). Consequently, this covers the four mandatory label minerals — Sodium, Calcium, Iron, and Potassium — as well as most other mandatory Nutrition Facts nutrients that require laboratory measurement.
Which of the mandatory FDA nutrients can AGT report under accreditation?
We cover most of them. Specifically, under our accredited scope we directly measure the four mandatory minerals (Sodium, Calcium, Iron, Potassium) plus Cholesterol, Protein, Total Fat, Saturated Fat and Trans Fat (via fatty-acid profile), Total Dietary Fiber, Total Sugars, and Vitamin D. Meanwhile, Calories and Total Carbohydrate are calculated from the measured analytes per FDA 21 CFR 101.9 convention rather than tested directly. Added Sugars cannot be determined in the laboratory at all — because analysis cannot separate added from naturally occurring sugars, that figure is documented from your formulation and ingredient records.
How is cholesterol measured, and can it support a "Low Cholesterol" claim?
Cholesterol is measured directly by GC-FID under AOAC 994.10 (SOP 080), not estimated from database values. As a result, the milligram-per-serving figure is defensible for the mandatory Nutrition Facts declaration and for nutrient content claims such as "Low Cholesterol" or "Cholesterol Free" under 21 CFR 101.62. In parallel, the AOAC 996.06 fatty-acid profile (SOP 079) supplies the saturated and trans fat values that those claims also depend on.
Do you test caffeine, and is it required on the label?
Yes, caffeine is quantified by HPLC under AOAC 979.08 / ISO 20481 (SOP 048) within our accredited scope. However, caffeine is not a mandatory Nutrition Facts nutrient. Instead, added caffeine must appear in the ingredient statement, and many brands additionally declare milligrams per serving on a voluntary basis. Accordingly, accredited data supports those voluntary declarations, decaffeinated claims, and batch consistency in coffee, tea, energy drinks, chocolate, and supplements.
What elements are included in the ICP-MS mineral panel?
In particular, the panel reports Sodium, Potassium, Calcium, Magnesium, Iron, Zinc, Copper, Manganese, Selenium, Chromium, Aluminium, Tin, and Nickel under AOAC 985.01 / 993.14 (SOP 047). Additionally, SOP 051 (AOAC 2015.06) extends coverage to Molybdenum. Furthermore, detection is at parts-per-billion sensitivity by ICP-MS, which is well below the levels required for label declarations.
Will my FDA Nutrition Facts label be defensible if challenged?
Yes, absolutely. Reports for our accredited nutrients are issued under PJLA ISO/IEC 17025:2017 accreditation (Cert L26-535). As a result, this is recognized through the ILAC mutual recognition arrangement and accepted by FDA inspectors and most retailer compliance teams as evidence of "Reasonable Care" in label generation.
What sample size do I need to send for nutrition testing?
Typically, we need 50–100 g of finished product for the mineral panel and 50–100 g or 250 mL for caffeine. On the other hand, cholesterol requires 100–200 g, and a full macronutrient and vitamin panel usually requires 200–300 g. However, for high-value or small-package products, smaller quantities can be accepted on consultation.

Need Reliable Nutritional Analysis and Food Labelling?

Join hundreds of food manufacturers who trust AGT Labs for accredited nutrient data on their Nutrition Facts labels.

Request a Nutrition Panel Quote
Scroll to Top