Shelf Life Testing &
Stability Studies

Scientifically determine your product's expiration date with accredited shelf life testing protocols. Accelerated and Real-Time stability programs anchored on PJLA-accredited Water Activity, pH, Peroxide Value, and Free Fatty Acid methods.

Accreditation
ISO/IEC 17025:2017 · Cert L26-535
PJLA-Accredited Stability Methods
Water Activity · pH · PV · FFA
Protocols
Accelerated & Real-Time
Validation
"Best By" / "Use By" Dates

Validate with a Trusted Shelf Life Testing Lab

AGT Food Labs provides accurate stability data so that you can determine how long your product maintains its quality, safety, and nutritional value. In short, whether you are launching a new product or changing packaging, our stability program produces the data needed to print "Best By" or "Expiration" dates with confidence.

To do this, we monitor the "Spoilage Trio" microbial growth, chemical degradation (rancidity), and organoleptic changes - to predict exactly when your product fails. Moreover, the chemical-stability anchors (Water Activity, pH, Peroxide Value, Free Fatty Acids, Titratable Acidity) are all run in-house under our PJLA scope.

Scope note: Our PJLA ISO/IEC 17025:2017 accreditation (Cert L26-535) covers the stability anchors used in shelf life testing - Water Activity (AOAC 978.18, SOP 044), pH (AOAC 981.12, SOP 036), Peroxide Value (AOCS Cd 8b-90, SOP 067), Free Fatty Acids (AOCS Ca 5a-40, SOP 063), Titratable Acidity (SOP 064), Aerobic Plate Count (AOAC 990.12), Yeast & Mold (AOAC 2014.05), and Vitamins A/C/D (HPLC), plus the 14-element ICP-MS mineral panel. By contrast, only sensory and physical (texture) analysis is coordinated through our partner laboratory network and consolidated into one shelf-life report.

Our Shelf Life Testing Process

From protocol design to the final report, we guide you through determining your product's shelf life.

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01. Protocol Design
First, we design a custom study (Frozen, Ambient, or High Heat / Humidity) based on your product type and target shelf life claim.
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02. Incubation
Next, samples are placed in humidity-controlled chambers for Real-Time or Accelerated aging at the agreed temperature/RH set point.
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03. Testing Pull Points
Then, at specific intervals (e.g., Week 0, 4, 8), we test PJLA-accredited Water Activity, pH, PV, FFA, APC, Yeast & Mold, and vitamins in-house; sensory / texture pulls are coordinated with our partner lab.
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04. Final Report
Finally, we analyze the data trends to scientifically validate your expiration date label and consolidate everything into one signed shelf life testing report.

Why Shelf Life Testing is Critical

For example, retailers like Walmart, Whole Foods, and Costco require scientific proof of your expiration dates. In fact, guessing your shelf life can lead to premature spoilage, costly recalls, and consumer illness.

Ultimately, a professional stability study not only ensures regulatory compliance but also protects your brand reputation by ensuring customers experience your product exactly as intended - from the first day to the last.

Our Shelf Life Testing Services

Comprehensive aging protocols matched to your timeline and product type.

Packaged food samples in a controlled chamber for accelerated shelf life testing
Fast Results

Accelerated Shelf Life Testing (ASLT)

Need to launch quickly? In that case, we use the Arrhenius Equation (Q10 Model) to predict shelf life. Specifically, by incubating samples at elevated stress (e.g., 35°C, 45°C, or 55°C), we increase the rate of chemical degradation to simulate months of aging in just weeks.

The Science: Based on a standard Q10 factor, we mathematically project your expiration date, allowing you to get to market faster while waiting for real-time validation.

Best For:
  • New Product Launches
  • Dry Goods (Low Water Activity)
  • Fats & Oils (PV / FFA tracking)
  • Rapid Prototyping
Packaged food samples stored under ambient warehouse conditions for real-time shelf life testing
Gold Standard

Real-Time Stability Studies

The only way to truly validate an accelerated prediction. Indeed, real-time shelf life testing stores your samples for the full duration under strict ICH Climatic Zone conditions (e.g., 25°C / 60% RH for ambient) to mirror your exact supply chain environment.

For this reason, we recommend running real-time studies in parallel with ASLT to confirm your label claims satisfy regulatory and retailer audits.

Best For:
  • Perishable Foods (Dairy, Meat)
  • Final Regulatory Validation
  • Frozen Products
The Spoilage Trio - microbial, chemical, and physical indicators tracked during shelf life testing
The Spoilage Trio

Microbial, Chemical & Physical Analysis

We don't just wait for mold. Instead, we use a holistic approach to define "End of Life" by monitoring the three pillars of quality degradation:

1. Microbial Spoilage: microbial spoilage indicators monitored over time - APC, Yeast and Mold - run in-house under our PJLA ISO/IEC 17025:2017 scope.
2. Chemical Rancidity & Nutrient Loss: chemical quality markers Peroxide Value (AOCS Cd 8b-90) and Free Fatty Acids (AOCS Ca 5a-40) tracked in-house before flavors turn bitter, alongside mineral and vitamin stability for label claims via our PJLA-accredited 14-element ICP-MS panel and Vitamin A/C/D profiling (HPLC).
3. Physical & Sensory: Texture Analysis to measure hardness, crispness, and separation so the product feels right in the consumer's mouth at end-of-life.
Parameters Tracked:
  • Chemical & Nutrient (PJLA): Water Activity, pH, PV, FFA, Titratable Acidity, Minerals, Vitamins A/C/D
  • Microbial (PJLA): APC, Yeast, Mold
  • Physical (Partner): Texture, Color Fading

Accelerated vs. Real-Time: Which Do You Need?

Ultimately, this side-by-side comparison helps you choose the right shelf life testing protocol for your timeline and product type.

Factor Accelerated (ASLT / Q10) Real-Time Stability
SpeedWeeks (months of aging simulated)Full shelf-life duration
Method BasisArrhenius / Q10 model at elevated temperature (35–55°C)ICH climatic-zone storage (e.g., 25°C / 60% RH)
Best ForNew launches, dry goods, fats & oils, rapid prototypingPerishables, frozen products, final regulatory validation
Regulatory WeightPredictive / provisional projectionGold-standard confirmation
Recommended UseRun first to reach market fasterRun in parallel to validate the label claim

Industries We Serve

Shelf life testing for the food & beverage categories where stability is the highest commercial risk.

Bakery and snack stability through accredited shelf life testing

Bakery & Snacks

Water Activity, mold, and texture tracking for breads, granola, and packaged snacks.

Dairy and beverage stability through accredited shelf life testing

Dairy & Beverages

pH, titratable acidity, and microbial trending for milk, yogurt, juice, and RTD drinks.

Sauces, dressings, and acidified foods stability through accredited shelf life testing

Sauces & Acidified Foods

pH, Water Activity, and FFA monitoring for dressings, sauces, and shelf-stable acidified products.

Nuts, oils, and high-fat foods stability through accredited shelf life testing

Nuts, Oils & Fats

Peroxide Value and Free Fatty Acid trending for nuts, oils, and high-fat snacks.

Analytical Parameters Monitored

Specifically, validated AOAC and AOCS methods are used to track degradation across each pull point.

Test Parameter Method Reference Scope Purpose in Shelf Life
Water Activity (Aw)AOAC 978.18, SOP 044✅ PJLA-AccreditedPredicts microbial stability & staling risk
pH StabilityAOAC 981.12, SOP 036✅ PJLA-AccreditedMonitors fermentation or spoilage shifts
Peroxide Value (PV)AOCS Cd 8b-90, SOP 067✅ PJLA-AccreditedMeasures initial oxidative rancidity in fats & oils
Free Fatty Acids (FFA)AOCS Ca 5a-40, SOP 063✅ PJLA-AccreditedMeasures hydrolytic rancidity (off-flavors)
Titratable AcidityAOCS Ca 5a-40, SOP 064✅ PJLA-AccreditedAcid balance & spoilage in dairy, sauces, beverages
Aerobic Plate Count (APC)AOAC 990.12✅ PJLA-AccreditedGeneral bacterial growth indicator
Yeast & MoldAOAC 2014.05✅ PJLA-AccreditedPrimary spoilage in acidic / dry foods
Vitamin Profiling (HPLC)AOAC 992.06 / 2012.22 / 2016.05✅ PJLA-AccreditedVerifies Vitamin A/C/D label claims over time

Industry Compliance

Meeting the strict requirements of retailers and regulators.

Retailer Standards

For instance, our shelf life testing reports meet the verification standards required by major retailers like Whole Foods, Walmart, Costco, and Amazon.

Challenge Studies

In addition, pathogen-inoculation challenge studies (Listeria / Salmonella) are coordinated through our partner microbiology network and integrated into the stability report.

Packaging Integrity

Meanwhile, we assess container-closure integrity to ensure seal failures do not compromise the product during its shelf life window.

Frequently Asked Questions

Common questions about Shelf Life and Stability Testing.

Which shelf life testing methods does AGT run in-house under PJLA accreditation?
Our PJLA ISO/IEC 17025:2017 scope (Cert L26-535) covers the stability anchors used in shelf life testing: Water Activity (AOAC 978.18, SOP 044), pH (AOAC 981.12, SOP 036), Peroxide Value (AOCS Cd 8b-90, SOP 067), Free Fatty Acids (AOCS Ca 5a-40, SOP 063), Titratable Acidity (SOP 064), Aerobic Plate Count (AOAC 990.12), Yeast & Mold (AOAC 2014.05), and Vitamins A/C/D by HPLC. By contrast, only sensory and physical/texture analysis is coordinated through our accredited partner network.
How long does shelf life testing take?
An accelerated study typically runs 6 to 12 weeks. For example, 1 week at 45°C simulates about 1 month of real-time aging, so you can validate a 12-month date in roughly 3 months.
How much does shelf life testing cost?
Cost depends on the study design - for example, the number of parameters, the pull points, and whether you run an accelerated study, a real-time study, or both. As a result, a short accelerated study is more economical than a multi-pull program that adds microbial, nutrient, and sensory panels. Therefore, share your product and target claim, and we'll send a custom quote.
How do I prepare and ship samples for a shelf life study?
First, send your product in its final retail packaging so that the study reflects real conditions, and provide enough units for every pull point plus retains. In addition, complete the Shelf Life Questionnaire and Chain of Custody (COC) form, and ship with the temperature control your product needs (ambient, refrigerated, or frozen). Finally, we'll confirm the exact sample count and schedule when we finalize your protocol.

Don't Guess Your Expiration Date.

Get scientific validation for your product with our accredited shelf life testing program.

Request a Stability Quote
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